Small Molecule Keeps Mutant SOD1 Out of Trouble

Mutations in copper-zinc superoxide dismutase 1 (SOD1) derail folding of the protein, preventing it from playing nice with its siblings. This leads the mutant down a path to aggregation and destruction, but an April 27 study in Nature Communications, claims to be on to a fix. Researchers led by Samar Hasnain at the University of Liverpool in England reported that the antioxidant ebselen promotes disulfide bonds in mutant SOD1, restoring its ability to fold properly and dimerize with other SOD1 proteins, steering away from aggregation. In human cells, ebselen treatment shifted levels of unfolded mutant SOD1 in favor of neatly folded proteins. Given that ebselen has already been tested for bipolar disorder and hearing loss, the researchers propose it could be quickly tested as a therapeutic to treat familial amyotrophic lateral sclerosis caused by SOD1 mutations.

It takes two. The antioxidant ebselen promotes the formation of disulfide bonds in mutant SOD1, restoring its ability to fold properly, and potentially reducing aggregation (see also Ray et al., 2004). [Courtesy of Capper et al., 2018, Nature Communications.]

“This is a novel folding and assembly corrector that could become a drug candidate,” commented Jeff Kelly of Scripps Research Institute in La Jolla, California.

Inclusions chock full of aggregated SOD1 proteins are the pathological hallmark of dying motor neurons in people with SOD1-ALS (Jan 2009 newsKerman et al., 2010). The disease likely begins with SOD1 misfolding, and the enzyme’s propensity to unfold and aggregate correlates with life expectancy of ALS patients and of mice that express the mutant human enzyme (see Oct 2014 news; Jonsson et al., 2006Seetharaman et al., 2009).

Disulfide bonds, as well as zinc and copper binding, are integral to SOD1 folding. First author Michael Capper and colleagues set out to restore those bonds. They screened a library of cysteine-reactive compounds for those that would oxidize cysteine residues in SOD1 and got two hits: ebselen and ebsulphur. Ebselen is an organoselenium compound with antioxidant and anti-inflammatory properties, which has been tested for treatment of multiple central nervous system disorders, including stroke, bipolar disorder, and hemorrhage (see Yamaguchi et al., 1998Singh et al., 2013Saito et al., 1998 ). It also bears a structural resemblance to edavarone, another antioxidant recently approved to treat ALS (see May 2017 news).

Structural analysis of the compounds bound to SOD1 revealed that ebselen and ebsulphur form covalent selenylsulfide or disulfide bonds, respectively, with cysteine-111 on SOD1. Using native mass spectrometry, the researchers found that the compounds promoted SOD1 dimerization and treating multiple dimerization-defective ALS mutants with ebselen or ebsulphur restored dimers to wild-type levels. The treatment had the strongest effect on the aggressive A4V mutant, strengthening its dimers by 60-fold. Ebselen or ebsulphur did not affect SOD1’s interaction with the chaperone hCCS, which is critical for its function. Overall, ebselen outperformed ebsulphur in promoting SOD1 dimerization, so the researchers used it for future studies.

In the Dimer Groove. Ebselen (ball and stick) bound to SOD1 dimers at cysteine-111 residues at the dimer interface. Left shows ribbon model, right shows electron density map. [Courtesy of Capper et al., 2018, Nature Communications.]

All this was done in solution. Does ebselen affect SOD1 folding and function in the complex chemical milieu of the cytosol? To find out, Capper assessed the state of SOD1 in HEK293 cells using in-cell NMR. Normally, the protein starts out with free cysteines and no disulfide bonds. Zinc, then copper, bind before disulfides form. The researchers found that in cells treated with ebselen, SOD1 was in its fully oxidized state, with an intramolecular disulfide bond formed between cysteine-57 and 146. The researchers proposed that ebselen transiently interacted with both these cysteines and catalyzed the formation of their disulfide bond. This is distinct from the Cys111-mediated dimerization ebselen evoked in vitro, and the authors attributed this difference to the highly reducing environment of the cellular cytosol.

Would this Cys57-Cys146 disulfide also stabilize SOD1 mutant proteins? Yes, according to in-cell NMR experiments on cells expressing G93A or A4V SOD1 mutants. In untreated cells, the mutant proteins either existed as unfolded monomers or were barely detected due to degradation. But ebselen boosted oxidation of both mutants, and restored their mature copper- and zinc-loaded versions to the levels seen for wild-type SOD1.

Overall, it appears ebselen stabilizes SOD1 in two ways, depending on the environment. In the less reducing environs of the test tube, ebselen formed a covalent bond with cysteine-111, which in turn promoted dimerization of the protein. In the cytosol, ebselen promoted a disulfide bond between SOD1 cysteines 57 and 146, which also stabilized the protein.

Though the researchers observed the cysteine-111 reaction only outside the cellular environment, they think it could still come into play in less strongly reducing compartments, such as the mitochondrial intermembrane space or extracellular matrix. Ebselen’s mechanism of action could also depend on dose, an idea currently being tested in SOD1-transgenic mouse models, Hasnain told Alzforum.

Featured Paper

Capper MJ, Wright GS, Barbieri L, Luchinat E, Mercatelli E, McAlary L, Yerbury JJ, O’Neill PM, Antonyuk SV, Banci L, Hasnain SS. The cysteine-reactive small molecule ebselen facilitates effective SOD1 maturation. Nat Commun. 2018 Apr 27;9(1):1693. PubMed.

References

Kerman A, Liu HN, Croul S, Bilbao J, Rogaeva E, Zinman L, Robertson J, Chakrabartty A. Amyotrophic lateral sclerosis is a non-amyloid disease in which extensive misfolding of SOD1 is unique to the familial form. Acta Neuropathol. 2010 Mar;119(3):335-44. PubMed.

Jonsson PA, Graffmo KS, Andersen PM, Brännström T, Lindberg M, Oliveberg M, Marklund SL. Disulphide-reduced superoxide dismutase-1 in CNS of transgenic amyotrophic lateral sclerosis models .Brain. 2006 Feb;129(Pt 2):451-64. PubMed.

Seetharaman SV, Prudencio M, Karch C, Holloway SP, Borchelt DR, Hart PJ. Immature copper-zinc superoxide dismutase and familial amyotrophic lateral sclerosis.Exp Biol Med (Maywood). 2009 Oct;234(10):1140-54. Epub 2009 Jul 13 PubMed.

Yamaguchi T, Sano K, Takakura K, Saito I, Shinohara Y, Asano T, Yasuhara H. Ebselen in acute ischemic stroke: a placebo-controlled, double-blind clinical trial. Ebselen Study Group. Stroke. 1998 Jan;29(1):12-7. PubMed.

Singh N, Halliday AC, Thomas JM, Kuznetsova OV, Baldwin R, Woon EC, Aley PK, Antoniadou I, Sharp T, Vasudevan SR, Churchill GC. A safe lithium mimetic for bipolar disorder. Nat Commun. 2013;4:1332. PubMed.

Saito I, Asano T, Sano K, Takakura K, Abe H, Yoshimoto T, Kikuchi H, Ohta T, Ishibashi S. Neuroprotective effect of an antioxidant, ebselen, in patients with delayed neurological deficits after aneurysmal subarachnoid hemorrhage. Neurosurgery. 1998 Feb;42(2):269-77; discussion 277-8. PubMed.

Further Reading

Ray SS, Nowak RJ, Strokovich K, Brown RH Jr, Walz T, Lansbury PT Jr. An intersubunit disulfide bond prevents in vitro aggregation of a superoxide dismutase-1 mutant linked to familial amytrophic lateral sclerosis. Biochemistry. 2004 May 4;43(17):4899-905. PubMed.

Wood-Allum CA, Barber SC, Kirby J, Heath P, Holden H, Mead R, Higginbottom A, Allen S, Beaujeux T, Alexson SE, Ince PG, Shaw PJ. Impairment of mitochondrial anti-oxidant defence in SOD1-related motor neuron injury and amelioration by ebselen. Brain. 2006 Jul;129(Pt 7):1693-709. PubMed.


To view commentaries, primary articles and linked stories, go to the original posting on Alzforum.org here.

Copyright © 1996–2018 Biomedical Research Forum, LLC. All Rights Reserved.

dimer dimerization disease-als disulfide bond ebselen misfolded SOD1 SOD1 topic-preclinical topic-randd
Share this:
Facebooktwittergoogle_plusmailFacebooktwittergoogle_plusmail